Introduction

Ozone therapy is a complementary medical treatment that uses a controlled mixture of ozone (O₃) and oxygen (O₂) to improve oxygen utilization, reduce microbial load, and support tissue healing.
It is used in integrative, naturopathic, and regenerative medicine for its antimicrobial, anti-inflammatory, and immunomodulatory effects

Different forms include:

  • Major Autohemotherapy (MAHT) – most common systemic method
  • Minor Autohemotherapy
  • Rectal Insufflation
  • Topical Ozone/Ozonated oils
  • Ozone Sauna (HOCATT)
  • Ozone injections (Prolozone)

Procedure

a. Major Autohemotherapy (MAHT) – Systemic Method

  • Patient sits or lies comfortably.
  • A measured amount of blood (usually 50–200 ml) is drawn into a sterile ozone-resistant bottle.
  • Medical-grade ozone–oxygen mixture is introduced at a specific concentration.
  • The blood is gently mixed and then reinfused into the patient.
  • Entire session lasts 20–40 minutes.

b. Rectal Insufflation – Non-invasive Method

  • Patient lies in left lateral position.
  • A small catheter is inserted 2–3 cm into the rectum.
  • Low-volume ozone gas mixture is introduced slowly.
  • Acts as a systemic treatment through absorption via the colon.

c. Local Applications

  • Ozonated oils/creams
  • Ozone bagging for wounds
  • Ozone steam sauna

Benefits

  • Antibacterial, antiviral, antifungal effects
  • Improves oxygen delivery to tissues
  • Reduces inflammation in chronic inflammatory conditions
  • Enhances wound healing (diabetic ulcers, infected wounds)
  • Modulates immune response
  • Pain reduction in musculoskeletal conditions (via Prolozone injections)
  • Improves circulation and microvascular function
  • May assist in detoxification by reducing oxidative stress when administered properly
  • Supportive in chronic conditions like fatigue, infections, ischemic diseases (adjunct therapy)

Contraindications

  • G6PD deficiency
    (Risk of hemolysis when exposed to oxidative therapies)
  • Active pregnancy
  • Uncontrolled hyperthyroidism
  • Significant bleeding disorders
  • Recent myocardial infarction
  • Ozone allergy/sensitivity (rare but reported)
  • Severe cardiovascular instability
  • Severe anemia
  • Acute alcohol intoxication
  • Febrile illness
  • Patients on blood thinners (need dose adjustment)
  • Chronic obstructive pulmonary disease (for inhalation risk—ozone should never be inhaled)